Fugene 6 transfection reagent is stabilized for extended storage. It does not contain any ingredients of human or animal origin. Storage and stability fugene 6 reagent is shipped at room temperature. Msds fugene 6 transfection reagent, 1814443 ethanol.
By clicking the download button on this page in order to start downloading the manual, you indicate your acceptance of this agreement and are agreeing to be bound by the terms of this agreement. In 6cmdish, when the confluence of 293t reachs 50%,i conduct the transfection. Airpods 2nd generation with wireless charging case quick start guide. We analyzed cell viability using the cell proliferation reagent wst1 roche applied science according to the instructions. Transfection of cultured eukaryotic cells using cationic lipid. Description fugene 6 transfection reagenta is a nonliposomal reagent that transfects dna into a wide variety of cell lines with high e. Instructions for use of products e2691, e2692, e2693. Helper free system can accommodate inserts of up to 3 kb, depending on the expression vector used. Fugene hd transfection reagent is a proprietary mixture of lipids and other components in 80% ethanol, sterile filtered and supplied in sterile glass vials. Pdf highefficiency nonviral transfection of primary. Fugene 6 transfection reagent is a nonliposomal formulation designed to transfect plasmid dna into a wide variety of cell lines with high efficiency and low toxicity.
Dnalipid complexes must be prepared in an antibiotic. Fugene 6 transfection reagent is a proprietary blend of lipids and other components supplied in 80% ethanol, sterilefiltered, and packaged in glass vials. User s manual english for customers in asia, oceania, the middle east. Highefficiency nonviral transfection of primary chondrocytes. A protocol for the fugene 6 transfection reagent, a nonliposomal reagent that transfects dna into a wide. Note that the instructions and plate layout in the spreadsheet need to be strictly followed. Viafect transfection reagent transfection assay e4981. All offer broad spectrum transfection capability and low toxicity. Fugene 6 transfection reagent protocol promega corporation. Transfection protocols often require serumfree conditions for optimal.
Fugene hd transfection reagent technical manualpdf 395 kb english. There are over 360,000 automotive manuals you can view for free. Promega offers 3 premium transfection reagents, fugene 6, fugene hd and viafect. Fugene 6 transfection reagent technical manualpdf 811 kb english. Transfection mixes are prepared by the addition of dna to 100. Oligofectamine invitrogen according to the manufacturers instructions.
The use of fugene hd does not require culture medium change or serum removal during transfection experiment. Always mix fugene 6 reagent prior to use vortex for one second or use inversion. Here we compared the transfection and viral transduction efficiency using commercially available transfection reagents including fugene 6. Allow fugene hd transfection reagent to reach room temperature, and mix. Cas 64175 fugene 6 transfection reagent, 1814443 ethanol msds toxicity property. If you need to download a manual there is also an option for this. An improved method for increasing the efficiency of gene.
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